The MtoZ Biolabs’ Co-Immunoprecipitation (Co-IP) Kit enables stable enrichment of target proteins and their interacting complexes under mild conditions, balancing specificity, recovery, and experimental reproducibility.
Product Overview
When performing protein immunoenrichment in complex biological samples, experimental outcomes are often influenced by multiple factors, making it difficult to achieve both high enrichment efficiency and reliable data quality.
1. Complex Sample Background
Cell lysates or tissue extracts contain large amounts of non-target proteins, which can lead to nonspecific adsorption and increased background interference.
2. Protein-Protein Interactions Are Easily Disrupted
Improper control of lysis and washing conditions may disrupt protein complex integrity, resulting in the loss of genuine interaction signals.
3. Variable Enrichment Efficiency
Differences in antibody quality, conjugation status, and experimental conditions can cause fluctuations in capture efficiency between experiments.
4. Limited Target Protein Recovery
Target proteins may be lost during washing and elution steps, affecting detection sensitivity and result consistency.
5. High Demands on Experimental Reproducibility
The multistep and parameter-sensitive nature of the workflow makes it difficult to obtain consistent and reliable results without a standardized system.
Advantages
1. Broad Compatibility
Suitable for a variety of cell and tissue samples and compatible with multiple downstream analytical methods.
2. High Versatility
Can be used with immunoprecipitation antibodies from different sources and of different types, supporting a wide range of applications.
3. Convenient Operation
A clear workflow and mild conditions reduce the need for extensive optimization and improve overall experimental success rates.
4. Reduced Antibody Interference
The design helps minimize nonspecific antibody binding and background interference, enhancing the reliability of enrichment results.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
