The Micro C-Trap Proteinase K Digestion and Desalting Kit from MtoZ Biolabs offers a streamlined, high-throughput solution for small-scale protein digestion using Proteinase K, followed by integrated desalting, providing purified peptides for mass spectrometry analysis with reliable and reproducible results.
Product Overview
Protein digestion and desalting are essential steps for preparing high-quality samples for mass spectrometry (MS) analysis, particularly when dealing with small or precious samples. Handling challenges can include:
1. Inconsistent Digestion
Without optimal conditions, digestion can be incomplete, leading to poor peptide yield and affecting the quality of MS data.
2. Contamination from Sample Matrix
Residual salts, detergents, and matrix impurities can interfere with MS analysis, causing background noise and reducing sensitivity.
3. Sample Loss During Handling
Multi-step protocols increase the risk of sample loss or contamination, especially when working with small sample volumes or low-abundance proteins.
4. Time-Intensive Procedures
The need for multiple steps often increases the complexity and time required for sample preparation.
Advantages
The MtoZ Biolabs Micro C-Trap Proteinase K Digestion and Desalting Kit offers the following advantages:
✅ Efficient Protein Digestion
Proteinase K cleaves proteins at hydrophobic and aromatic residues, ensuring complete digestion and high peptide yield for MS analysis.
✅ Integrated Desalting Step
The kit’s built-in desalting step removes salts, detergents, and other impurities, ensuring clean peptides suitable for high-sensitivity MS analysis.
✅ Optimized for Small-Scale Applications
The microplate format is ideal for processing low-volume samples, ensuring efficient peptide recovery while minimizing sample handling.
✅ Streamlined Workflow
The integrated digestion and desalting process eliminates sample transfers, reducing handling time and minimizing errors or sample loss.
✅ Reproducible and Reliable Results
With standardized protocols, the kit ensures consistent and reproducible results, ideal for proteomics research.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
