MtoZ Biolabs' HLA Class II Immunopeptide Enrichment Kit is designed to isolate HLA class II bound peptides from cells and tissues for LC-MS based immunopeptidomics, neoantigen discovery, and antigen presentation studies.
Product Overview
Enriching HLA class II immunopeptides from biological samples is challenging because the ligands are low abundance, heterogeneous, and embedded in a complex protein matrix. Key difficulties include:
1. Low Abundance on Cell Surfaces
HLA class II complexes are present at limited copy numbers on many primary cells and tissue samples, so direct analysis without enrichment yields very few identifiable class II peptides.
2. High Background of Non HLA Peptides
Cell lysis and protein digestion generate large amounts of unrelated peptides from abundant intracellular proteins, which easily overwhelm HLA class II ligands in LC-MS runs.
3. Heterogeneous Peptide Length and Binding
HLA class II molecules present peptides of variable length with open ended binding grooves, and harsh enrichment or elution conditions can bias recovery toward only a subset of the true immunopeptidome.
4. MS Incompatible Buffers and Detergents
Detergents and chaotropes commonly used for membrane solubilization and immunoprecipitation can interfere with chromatography and ionization, reducing sensitivity and data quality in downstream LC-MS analysis.
Advantages
✔️High Target Specificity
The HLA Class II Immunopeptide Enrichment Kit from MtoZ Biolabs uses class II directed capture reagents and an optimized IP workflow to selectively pull down HLA class II complexes, greatly reducing co isolation of unrelated proteins and non HLA peptide background.
✔️High Sensitivity Peptide Recovery
Enrichment and elution conditions are tuned to recover low abundance HLA class II bound peptides with high efficiency, improving detection of rare ligands and neoantigen candidates in LC-MS based immunopeptidomics.
✔️Broad Sample Compatibility
The workflow accommodates a wide range of input materials, including cell lines, primary immune cells, PBMCs, and frozen or fresh tissues, allowing consistent HLA class II peptide profiling across diverse experimental and clinical sample types.
✔️MS Friendly Workflow Compatibility
MtoZ Biolabs formulates the HLA Class II Immunopeptide Enrichment Kit with MS compatible buffers and peptide elution conditions, minimizing detergent and chaotrope carryover so enriched ligands can be injected directly into LC-MS workflows with reliable chromatographic performance and signal quality.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
