MtoZ Biolabs HLA Class I Immunopeptide Enrichment Kit - Engineered for High-Sensitivity and High-Specificity Isolation of HLA Class I - Presented Antigenic Peptides to Enable Comprehensive Profiling of Tumor Neoantigens and Immune Surveillance Landscapes
Product Overview
In tumor immunology and vaccine development, the precise characterization of endogenous antigen peptides presented by HLA Class I molecules is critically important. However, due to the extremely low abundance and strong hydrophobicity of these peptides, as well as substantial interference from high-abundance serum proteins, conventional enrichment strategies encounter significant technical challenges:
1. Insufficient Specificity and Background Interference
Conventional enrichment approaches exhibit limited capture specificity toward HLA Class I molecules, leading to the co-enrichment of substantial amounts of non-target peptides and proteins, which severely obscures authentic antigen peptide signals.
2. Extremely Low-Abundance Peptide Recovery Is Difficult
Target antigen peptides are typically present at extremely low levels, and the binding efficiency of conventional enrichment matrices is insufficient to enable effective capture, resulting in the loss of critical immunological information.
3. Limited Sample Compatibility and Throughput
The processing efficiency varies across samples from different sources (such as cell lines and tissues) and under different storage conditions, making it difficult to meet the requirements for diverse sample types and high-throughput analysis in clinical studies.
4. Interference with Downstream Mass Spectrometry Analysis
Salts, detergents, or dissociated affinity ligands introduced during the enrichment process may severely suppress ionization efficiency in mass spectrometry, thereby compromising both identification sensitivity and analytical accuracy.
Advantages
✅ Superior Targeting Specificity
Validated antibodies with high affinity and high specificity are employed in the MtoZ Biolabs HLA Class I Immunopeptide Enrichment Kit to precisely capture intact HLA Class I molecule–peptide complexes. The non-specific binding background is extremely low, thereby ensuring the reliability of the data and a high signal-to-noise ratio.
✅ Ultra-High Sensitivity Recovery
Optimized reaction conditions combined with solid-phase supports maximize binding efficiency, enabling efficient enrichment of extremely low-abundance antigenic peptides and maximizing the depth of immunopeptidomic information obtained from the samples.
✅ Broad Sample Compatibility
Following rigorous validation, the method enables efficient processing of multiple sample types, including cultured cells as well as fresh and frozen tissues, thereby providing a powerful platform for translational medicine research.
✅ Excellent Compatibility With Mass Spectrometry Workflows
The enriched peptide samples exhibit high purity, with key interfering substances efficiently removed, allowing direct application to high-resolution liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis and ensuring the acquisition of high-quality spectral data.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
