The High pH Reversed-Phase Peptide Fractionation Column from MtoZ Biolabs is designed to introduce an orthogonal high pH fractionation step that reduces sample complexity before downstream LC-MS analysis and enhances proteome depth.
Product Overview
Deep proteome sequencing often benefits from fractionation, but routine fractionation steps can introduce some challenges.
1. Limited Depth
Single-shot runs can miss low-abundance peptides, especially in complex digests, making coverage and pathway discovery incomplete.
2. Poor Orthogonality
Fractionation that is not sufficiently different from low pH LC can yield overlapping fractions, reducing the practical gain in identifications.
3. Limited Sample Compatibility
Standard fractionation methods may not work well for complex samples, such as phosphorylated or Tandem Mass Tag (TMT)-labeled peptides, limiting their applicability in diverse proteomic studies.
4. Reproducibility Issues
Inconsistent fractionation results between runs or across experiments can make it difficult to compare data or scale studies, especially in large proteomics projects.
Advantages
✅ Efficient Peptide Fractionation
High-pH reversed-phase chromatography efficiently separates peptides by hydrophobicity, offering a robust orthogonal fractionation method that complements traditional low-pH reversed-phase LC-MS gradients.
✅ Increased Protein Sequence Coverage
By improving peptide separation, the column enhances sequence coverage and increases protein identification rates, helping to uncover deeper insights into the proteome.
✅ No Additional Desalting Required
Fractionated peptides are ready for direct analysis by LC-MS without the need for extra desalting steps, simplifying the workflow and saving time.
✅ Broad Sample Compatibility
Suitable for a variety of peptide mixtures, including native, phosphorylated, and TMT-labeled peptides, enabling flexible proteomic analysis.
✅ High Reproducibility
MtoZ Biolabs' High pH Reversed-Phase Peptide Fractionation Column ensures consistent results across different experiments, providing reliable fractionation and enhancing reproducibility for large-scale studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
