MtoZ Biolabs' C18 Peptide Desalting Spin Column is a ready-to-use cleanup tool for fast peptide desalting and contaminant removal prior to LC-MS/MS analysis, improving signal quality without a complicated workflow.
Product Overview
Peptide desalting may seem straightforward, but routine peptide cleanup methods can introduce practical challenges when you need fast processing, high recovery, and consistent results.
1. Complex Operation
Conventional peptide cleanup often relies on multi-step procedures that require specialized setups and repeated sample transfers. This not only slows down throughput but also increases the risk of handling errors and variability.
2. Peptide Loss
When processing low-amount samples, peptides can be lost during the desalting workflow due to insufficient binding, wash loss, or incomplete elution, resulting in low recovery and compromising downstream identification and quantification.
3. Carryover of MS-Incompatible Reagents
Residual salts, detergents, or buffers can suppress ionization, distort chromatographic peaks, and reduce sensitivity.
4. Limited Condition Tolerance
Some cleanup media perform inconsistently with labeled or complex peptide mixtures or under very acidic or basic conditions, leading to unstable retention and uneven results.
Advantages
✔️Convenient Format
MtoZ Biolabs' C18 Peptide Desalting Spin Column comes prepacked as a single-use spin unit that drops into standard 2 mL microcentrifuge tubes, simplifying setup and minimizing hands-on steps.
✔️High Recovery
Our hydrophobic C18 resin supports strong peptide retention and clean elution, improving usable recovery across a wide peptide load range.
✔️Cleaner MS Readout
Efficient removal of salts and other small-molecule contaminants improves signal-to-noise and overall spectral quality for downstream analysis.
✔️Broad Compatibility
Designed for demanding proteomics workflows, this column effectively purifies peptides from complex samples, including those modified with isobaric tags (e.g., TMT, iTRAQ). Its performance is stable across a broad pH range, enabling flexible optimization of wash and elution conditions.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
