Resources
Proteomics Databases
Metabolomics Databases
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• How to Analyze Histone Acetylation Sites by Mass Spectrometry?
In epigenetic research, histone acetylation is a key post-translational modification (PTM) that regulates chromatin accessibility and gene expression. The composition and dynamic changes of acetylation sites directly reflect the transcriptional regulatory state of cells. Therefore, achieving high-sensitivity and high-coverage analysis of acetylation sites has become a critical technical challenge at the intersection of proteomics and epigenetics. Mass spectrometry has emerged as one of the most powerf......
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• Lactylation Proteomics Analysis: A Comprehensive Workflow from Enrichment to Identification
Lactylation (Lysine Lactylation, Kla) is an important post-translational modification discovered in recent years and has been demonstrated to play critical roles in gene expression regulation, immunometabolism, tumor biology, and related fields. However, as a low-abundance and technically challenging acylation modification, lactylation research imposes higher demands on experimental methodologies. How can lactylation sites be accurately captured from complex proteomes? How can high-throughput, high-re......
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• How to Perform Histone Propionylation Proteomics Analysis?
Histone propionylation is an important post-translational modification that plays a critical role in regulating gene expression, chromatin remodeling, and the pathogenesis of various diseases. With advances in proteomic technologies, researchers can systematically characterize the distribution and functional implications of histone propionylation using high-throughput proteomic approaches, providing valuable data for precision medicine research. Scientific Background of Histone Propionylation Proteom......
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• How to Detect Low-Abundance Histone Propionylation?
Histone propionylation is a critical epigenetic modification that plays an important role in gene regulation. However, its cellular abundance is typically low, posing significant challenges for detection and quantification. To address this issue, researchers have developed a range of reliable detection methods by integrating highly sensitive mass spectrometry techniques, peptide enrichment strategies, and optimized experimental designs. Challenges in Detecting Low-Abundance Histone Propionylation His......
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• How to Apply Exosome Proteomics in Cancer Research?
With the advancement of precision medicine, cancer research is increasingly transitioning from single-cell analysis to multi-omics studies of biofluids. Exosomes, as natural carriers of intercellular information, hold significant potential in early cancer diagnosis, identification of therapeutic targets, and investigations of the tumor microenvironment through their proteomic content. Definition of Exosomes and Their Significance in Cancer Research Exosomes are small vesicles, approximately 30-150 nm......
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• How Is Protein Extraction Performed from FFPE Tissue for Mass Spectrometry?
Formalin-Fixed Paraffin-Embedded (FFPE) tissue is the most widely used method for sample preservation in clinical and basic research. Due to their stability during long-term storage and the wealth of associated clinical information, FFPE samples have become an essential resource in histology, pathology, and biomarker research. However, protein extraction from FFPE tissue for mass spectrometry remains a major technical challenge in proteomics studies. Challenges in Protein Extraction from FFPE Tissue ......
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Glycosylation is one of the most widespread post-translational modifications in eukaryotic proteins and plays integral roles in cellular communication, receptor recognition, immune regulation, tumorigenesis, and other biological processes. As two major forms of glycosylation, N-glycosylation and O-glycosylation share the same broad modification category but differ substantially in modification mechanisms, structural diversity, and analytical strategies. This article systematically compares the quantit......
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Glycosylation is one of the most widespread protein post-translational modifications and plays critical roles in multiple biological processes, including cellular recognition, signal transduction, and immune regulation. Glycoproteomic research not only facilitates a deeper understanding of the intricate regulatory mechanisms of biological systems but also shows substantial potential in elucidating disease mechanisms and discovering biomarkers. Advances in mass spectrometry have provided essential tech......
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• PhIP-Seq: What It Is and When Researchers Should Use It
Phage immunoprecipitation sequencing, usually referred to as PhIP-Seq, was developed for this type of question. Instead of testing one antigen at a time, PhIP-Seq uses large phage-displayed peptide libraries to profile antibody binding at scale.
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• Applications of iTRAQ-Based Peptidomics in Inflammation Research
iTRAQ is an isobaric isotope-labeling strategy that covalently labels peptide N-termini and lysine side chains. Through 4-plex or 8-plex multiplex labeling, iTRAQ enables simultaneous relative quantification of multiple samples. In tandem mass spectrometry (MS/MS), these isobaric tags fragment to generate characteristic reporter ions, allowing accurate comparison of peptide abundance across different treatment groups. iTRAQ-based peptidomics has been widely applied in inflammation research, including ......
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