Resources

• Identifying Protein Targets of Small Molecules: Study Strategies and Validation

Compare small-molecule target discovery strategies, learn how to prioritize candidate proteins, and explore binding assays for further validation.

• Protein-Ligand Interaction Analysis: How to Assess Binding and Interpret Results

A decision guide to affinity, kinetics, thermal stability, and conformational readouts for a known protein-ligand pair.

• RNA Pull-Down Assay: Protein Identification, Controls, and Interpretation

A guide to RNA bait design, biological context, negative controls, mass spectrometry candidate ranking, and validation.

• RNA Immunoprecipitation (RIP): Principles, Experimental Design, and Results

A practical guide to RIP method selection, antibody and sample quality, controls, RIP-qPCR, RIP-Seq, and evidence limits.

• Protein-RNA Interaction Analysis: Methods, Design, and Interpretation

A decision guide to RNA-centered, protein-centered, chromatin, and proximity strategies for protein-RNA interaction studies.

• ChIP-Seq for Protein-DNA Interaction Analysis: Principles, Design, and Interpretation

A practical guide to ChIP-Seq target selection, controls, data quality, peak interpretation, and evidence limits.

• Protein-DNA Interaction Analysis: Identifying Binding Proteins and DNA Targets

A guide to DNA-centered and protein-centered strategies for identifying associated proteins and genomic occupancy.

• Cross-Linking Mass Spectrometry (XL-MS) for Protein Interaction Mapping

Discover how cross-linking mass spectrometry identifies spatially linked residues and helps researchers investigate protein interaction interfaces and complex architecture.

• Beads, Eluate or Gel Band? How to Submit IP and Pull-Down Samples for Mass Spectrometry

Choose beads, eluate, or gel band for IP and Pull-Down LC-MS/MS submission by recovery, residual reagents, controls, and the intended identification question.

• Co-IP vs Pull-Down vs Proximity Labeling: Choosing a Protein Interaction Method

Compare Co-IP, Pull-Down, and proximity labeling by material, context, and evidence type. Choose a starting route from the research claim.