Resources

Proteomics Databases



Metabolomics Databases

• • Recent Advances in N-Terminal Protein Sequencing

  Protein N-terminal sequencing is the process of determining the amino acid sequence at the N-terminus of a protein or peptide chain. This is crucial for protein identification, analysis of signal peptide cleavage sites, and post-translational modification studies, among other areas. Recent advances in the field have been seen, particularly in the application of mass spectrometry and bioinformatics.

• • The Basic Processing Procedure of Protein Sequencing Sample

  Protein sequencing typically refers to the determination of the precise order of amino acid residues in a protein molecule, which is a crucial step in understanding protein function and structure. The main technique used to achieve this is mass spectrometry analysis, particularly tandem mass spectrometry (MS/MS). The following are the basic processing steps for protein sequencing.

• • Protein Mutation Site Analysis

  Mutation site analysis of proteins is an important biological research that focuses on specific changes that occur in the amino acid sequence of proteins, which may affect the function, stability, and/or interaction with other molecules of the protein. The following are basic concepts and methods of protein mutation site analysis.

• • Peptide Mass Spectrometry Result Interpretation Strategy

  Mass spectrometry (MS), is an important technique for analyzing peptides or proteins. It determines the molecular weight and structure by measuring the mass-to-charge ratio (m/z) of the analyte ions. In proteomics, MS is commonly used for protein and peptide identification, as well as quantitative analysis. To interpret the mass spectrometry results for peptide analysis, it is necessary to have a basic understanding of the mass spectrum, including m/z (mass-to-charge ratio) and intensity.

• • Protein Motif Analysis

  Protein motif analysis is an important task in bioinformatics, aiming to identify short sequence patterns in protein sequences that have specific functional or structural significance. These short sequences, often referred to as motifs or functional sites, are crucial for understanding the biological functions of proteins, protein-protein interactions, and regulatory mechanisms.

• • Methods and Applications for Protein Sequence Determination

  Protein sequencing is the process of determining the exact order of amino acids in a protein. It can analyze amino acid sequences, confirm protein structure and function, identify new protein biomarkers and drug targets, construct phylogenetic trees to understand evolutionary relationships, and identify orthologous and paralogous proteins.

• • How to Analyze Protein Multiple Sequence Alignment Results

  Multiple Sequence Alignment (MSA) is a technique to arrange three or more protein sequences in order to identify their similarities and differences to the maximum extent. Analyzing the results of protein MSA can reveal evolutionary relationships, structural domains, functional sites, and other important biological information among protein family members.

• • Overview of Single-Cell Protein Sequencing

  Single-cell proteomics is a method for studying the protein expression and regulation of individual cells. This technique is crucial for understanding cellular heterogeneity, revealing how different proteins interact within cells, and how they respond to external stimuli. Single-cell proteomics provides more detailed and accurate information because it directly measures the functional unit of cells - proteins.

• • De Novo Protein Sequencing: Basic Concepts and Applications

  De novo protein sequencing refers to determining the amino acid sequence of a protein without relying on known protein databases. Unlike other analysis methods that depend on known protein sequences or mass spectrometry databases, de novo sequencing directly analyzes peptide fragments using tandem mass spectrometry. Therefore, peptides that are not included in protein databases, protein sequences from new species, and protein sequences that have not been sequenced in the genome can all be analyzed.

• • N-Terminal Sequencing: Methods and Applications

  N-terminal sequencing is a technique used to determine the amino acid sequence of the N-terminal (amino-terminal) of a protein or peptide. This technique is useful in the field of biochemistry and molecular biology, particularly when researchers want to confirm the starting position of a protein or determine if a specific protein has undergone a particular modification.