Resources

Proteomics Databases



Metabolomics Databases

• • How to Detect Abnormal Glycoproteins

  Glycoproteins, also known as glycoproteins, are molecules that have one or more sugar chains attached to their protein structure. The structure and composition of these sugar chains play important roles in many biological processes, including cell recognition, signal transmission, and immune response.

• • Protein Full Length Sequencing

  Full-Length Protein Sequencing refers to determining the complete amino acid sequence of a protein from the N-terminus to the C-terminus. This process involves a series of techniques and is crucial for understanding the biological function of proteins, studying protein-protein interactions, and developing new drugs. Common methods for full-length protein sequencing include Mass Spectrometry (MS), protein sequencing analyzers, and bioinformatics methods.

• • Dia Protein Group Sequencing

  DIA (Data Independent Acquisition) protein sequencing is used for high-throughput and high-sensitivity protein analysis. Compared to traditional Data Dependent Acquisition (DDA) technology, DIA can provide more comprehensive protein coverage and more reliable quantitative results. DIA technology has a wide range of applications in biomedical research, biomarker discovery, and drug development.

• • Analysis of the N-Terminal Sequence of a Protein

  Protein synthesis starts from the N-terminus of a protein, and the composition of the N-terminal sequence of the protein influences its overall biological function. For example, the N-terminal sequence affects the half-life of the protein and is related to the protein's subcellular localization. N-terminal sequencing analysis of proteins helps in analyzing their higher-order structure and revealing their biological function.

• • Antigen Antibody Epitope Analysis

  The interaction between antigens and antibodies is central to immune response. This specific interaction occurs between the variable region of the antibody and a specific part of the antigen, which is called an "epitope". The binding of the antibody to its corresponding epitope is highly specific.

• • Antibody Drug Activity Testing

  Antibody drugs, especially monoclonal antibodies (mAbs), have played a crucial role in the treatment of many diseases, particularly cancer and immunological disorders. To ensure the effectiveness and safety of these antibody drugs, their activity needs to be assessed.

• • Dynamic Light Scattering Particle Analyzer: Principles and Uses

  Dynamic light scattering (DLS), also known as laser light scattering, is a technique used to measure the size of small particles (such as proteins, nanoparticles, polymers, or liposomes) in solution or suspension. DLS is based on analyzing the fluctuation of scattered light intensity from particles in a solution over time. When a laser beam is shone onto the sample, the particles in the sample scatter light.

• • IR Spectroscopy for Protein Secondary Structure

  Infrared spectroscopy, especially Fourier transform infrared spectroscopy (FTIR), is a commonly used technique for studying the secondary structure of proteins. This method is based on the vibrational absorption of the peptide bond C=O (amide I) and N-H (amide II) in different protein secondary structure components, such as α-helices, β-sheets, β-turns, and random coils.

• • Detection Methods for Host Cell Residuals in Antibody Drugs

  Monoclonal antibody drugs are produced using biotechnology, typically within host cells such as mammalian cells (e.g. CHO cells) or bacterial cells (e.g. E. coli). During the production process, there is a possibility of residual host cell contaminants entering the final drug product. These contaminants may include DNA, proteins, or other small molecules, which could potentially impact the safety and/or efficacy of the drug.

• • ADA Detection: Sample Collection, Processing, and Analysis

  "Antidrug antibodies" (ADA) usually refers to antibodies produced by the immune system against certain drugs, particularly biologics. When patients receive certain biologic therapies, such as monoclonal antibody therapy, their immune system may produce these antibodies, which can affect the safety and efficacy of the drugs. ADA testing is performed to determine whether these antidrug antibodies are present in the patient's body.