Resources

Proteomics Databases



Metabolomics Databases

• • Workflow of Protein Sample Preparation

  Protein sample preparation is a critical step in biological research. High-quality protein samples are essential for subsequent biochemical analyses, structural studies, and functional investigations. The following sections detail the workflow for protein sample preparation, including protein extraction, purification, quantification, and quality control.

• • Principle of Protein Sample Preparation

  Protein sample preparation is a critical step in biological research, directly impacting the accuracy and reliability of subsequent experiments. To obtain high-quality protein samples, scientists must adhere to a series of strict operational steps and principles. This article will detail the fundamental principles and related methods of protein sample preparation.

• • Differences of Labeled and Label-free Quantitative Proteomics

  Quantitative proteomics in proteinomics mainly involves two major strategies: labeled quantification and label-free quantification, which are used to compare protein abundance in different samples or under different treatment conditions.

• • Determination of Protein Disulfide Bonds

  The structure and function of proteins largely depend on their three-dimensional structure, and disulfide bonds, as one of the important covalent bonds in proteins, play a crucial role in maintaining the stability and functionality of proteins. Therefore, accurate determination of protein disulfide bonds is of great significance for understanding the structure and function of proteins.

• • Protein Phosphorylation Detection: Methods and Applications

  Protein phosphorylation is an important post-translational modification process, involving the addition of a phosphate group to a specific amino acid residue of a protein, typically serine, threonine, or tyrosine residue. Protein phosphorylation plays a critical role in regulating various biological processes within cells, including signal transduction, cell cycle, and metabolism.

• • Proteomic Identification of Human Cell Proteins

  The cell is the basic unit of life, and proteins are the key players in cell function and structure. In order to gain a deep understanding of cell function, growth, division, and their relationship with diseases, comprehensive identification and functional analysis of proteins are crucial. In this regard, mass spectrometry (MS) technology provides powerful tools for proteomics.

• • Determination of Primary Structure of Fish Tissue Protein

  As one of the main sources of food for humans, fish meat is rich in protein and has high nutritional value. In-depth research on the primary structure of proteins in fish meat not only helps to understand the nutritional composition and biological activity of fish meat, but also has important implications for food science, aquaculture, and pharmaceutical research.

• • Vitreous Humor Tissue Protein 2D Electrophoresis Analysis

  The vitreous humor tissue is a crucial tissue in the eye and plays a vital role in the normal functioning of the visual system. It contains various proteins that are closely related to eye diseases, aging, and other physiological processes. Therefore, the identification and quantification analysis of its proteins are of great significance.

• • Comparison of Proteomics Analysis

  Comparative proteomics is a branch of proteomics that involves comparing protein expression in different biological samples, such as cells in healthy and diseased states or cells from different species. This comparison can reveal which proteins have changed under specific conditions, helping scientists understand disease mechanisms, identify biomarkers, and even discover new therapeutic targets.

• • Drug Antibody Testing

  Drug antibody testing is a laboratory testing method specifically designed to evaluate the immune response of the human body to certain drugs. This test is commonly used to monitor a patient's immune response to biological agents or other drugs, especially in the treatment of chronic diseases such as autoimmune diseases, certain cancers, and inflammatory diseases. The principle of drug antibody testing is based on the recognition and response of the immune system to drug molecules.