Resources

Proteomics Databases



Metabolomics Databases

• • How to Detect the Level of Histone Methylation?

  Histone methylation refers to the process of adding methyl groups to specific lysine (Lys) and arginine (Arg) residues on histone proteins. This is a common epigenetic modification that has significant effects on chromatin structure and gene expression regulation. Detecting the level of histone methylation is one of the fundamental techniques in the study of epigenetics. Here are some commonly used detection methods:

• • Label-Free Proteomics

  Label-free proteome typically refers to a group of proteins in proteomics research that does not rely on any form of chemical or isotopic labeling for protein quantification analysis. Their analysis and quantification depend on label-free techniques, such as mass spectrometry-based relative quantification analysis.

• • Plasma Protein Profiling

  Plasma proteomics is an emerging scientific field that aims to analyze and understand the protein composition and function in plasma. Plasma, as one of the most abundant biological fluids in the human body, contains thousands of proteins that reflect the body's health status, disease processes, and treatment responses. Therefore, plasma proteomics holds great potential for disease diagnosis, treatment, and prognosis assessment.

• • DIA Data Analysis Methods in Proteomics

  Data-Independent Acquisition (DIA) is a mass spectrometry technique used for high-throughput, deep analysis of the proteome in complex biological samples. Compared to the traditional Data-Dependent Acquisition (DDA) method, DIA provides a more comprehensive and reproducible proteome analysis.

• • Application and Analysis of TMT Technology in Proteomics

  TMT (Tandem Mass Tags) technology is an advanced method used for protein quantification analysis in proteomics research. TMT labeling involves the use of a series of chemically distinct tags with different weights that can be attached to the amino terminus and side chains of peptides. This allows for simultaneous processing of multiple samples which are then combined for mass spectrometry analysis.

• • Proteomics Markers and Non-Markers

  Proteomics is the study of the complete set of proteins within a cell, including their expression, function, and regulation. In proteomics research, "labelled" and "unlabelled" are two main approaches.

• • Circular Dichroism: Analyzing Chiral Molecules and Biomolecule Structures

  Circular dichroism spectroscopy is an experimental method used to analyze molecular structure and chirality properties, commonly applied in the fields of chemistry, biochemistry, and biophysics. This technique utilizes the optical activity of substances when interacting with circularly polarized light to obtain information.

• • Host Protein Residue Detection: Key to Biopharmaceuticals

  Host protein residual detection refers to the detection of proteins derived from the production cell line (i.e., host) that may remain in the final product during the biopharmaceutical production process. This type of detection is crucial because these host cell proteins (HCPs) may affect the safety, efficacy, and quality of the drug product.

• • HCP Residual Detection: Ensuring Biopharmaceutical Safety and Quality

  HCP (Host Cell Proteins) residual detection is a critical quality control step in the field of biopharmaceuticals, aimed at ensuring that the levels of host cell proteins in the final drug product remain at acceptable low levels in any cell-based production system used to produce therapeutic proteins.

• • Nasopharyngeal Carcinoma Tissue MRM/PRM Quantitative Proteomic Analysis

  Nasopharyngeal carcinoma is a malignant tumor originating from the epithelium of the nasopharyngeal mucosa. Its incidence and mortality rates vary significantly geographically, particularly more common in Southeast Asia. Early diagnosis and treatment of nasopharyngeal carcinoma are crucial, however, due to the specific anatomical location of the nasopharynx, early symptoms are not obvious, often leading to late-stage detection of the disease.