Quantitative Enzyme Activity Analysis by ABPP and LC-MS/MS

Incubate lysates with ABPs, enrich tagged enzymes, digest, and quantify probe-modified peptides by label-free or TMT LC-MS/MS across conditions.

Key Takeaways

• Only active enzymes react with warheads.
• Include competition controls.
• Probe class defines enzyme coverage.
• MS quant tracks activity changes under drug treatment.

Related Services

Activity-Based Protein Profiling (ABPP) Service

Small Molecule Drug Targets Identification Service | ABPP

FAQ

1. ABPP vs expression proteomics?

ABPP reports catalytic activity, not total protein level.

2. What are limitations?

Probe design and non-covalent enzymes need tailored strategies.

Conclusion

Quantitative ABPP connects functional enzyme states to phenotypes and compound action.