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Plant Hormone Analysis Service

Plant hormones, also named phytohormones, are chemicals that regulate plant growth. Opposite to animal hormones, plant hormones can be synthesized and secreted by every plant cell. Plant hormones not only regulate local cellular activities, and can also be transported to other part of the plant. Hormone receptors are highly sensitive in detecting hormones, therefore, hormones usually occur in extremely low concentration. Thus detection of hormones requires highly sensitive technology. MtoZ Biolabs has establish a platform consisting of NMR and ACQUITY UPLC/TripleQuad5500 (Waters/AB Sciex), which is able to detecting extremely low amount of plant hormones with high accuracy. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be hugely reduced, thus further increasing detecting sensitivity.

 

Table 1. List of Plant Hormones Detectable by MtoZ Biolabs

NO.

Metabolite

NO.

Metabolite

NO.

Metabolite

1

Abscisic acid

44

cis-Zeatin

87

Brassinolide

2

ABA-glucosyl ester

45

Dihydrozeatin-7-glucoside

88

24-epi-Brassinolide

3

Indole-3-acetyl glutamic acid

46

Dihydrozeatin

89

28-norbrassinolide

4

3-Indoleacetonitrile

47

N6-isopentenyladenine

90

28-homobrassinolide

5

Indole-3-acetyl-L-glutamic acid dimethyl ester

48

N6-isopentenyladenosine

91

Castasterone

6

Indole-3-acetyl-L-leucine methyl ester

49

trans-Zeatin

92

6-deoxocastasterone

7

Indole-3-acetyl-L-valine methyl ester

50

trans-Zeatin riboside

93

28-norcastasterone

8

Indole-3-acetyl glycine

51

1-Aminocyclopropanecarboxylic acid

94

28-homocastasterone

9

2-oxindole-3-acetic acid

52

Gibberellin A1

95

6-deoxo-24-epi-Castasterone

10

Indole-3-acetyl-L-aspartic acid

53

Gibberellin A15

96

Typhasterol

11

N-(3-Indolylacetyl)-L-leucine

54

Gibberellin A19

97

28-norteasterone

12

N-(3-Indolylacetyl)-L-valine

55

Gibberellin A20

98

Kinetin-9-glucoside

13

Indole-3-acetyl-L-phenylalanne methyle ester

56

Gibberellin A24

99

2-Methylthio-N6-isopentenyladenine

14

Indole-3-acetyl-L-tryptophan

57

Gibberellin A3

100

2-Methylthio-cis-zeatin

15

3-Indole acetamide

58

Gibberellin A4

101

2-Methylthio-cis-zeatin riboside

16

Tryptamine

59

Gibberellin A53

102

2-Methylthio-N6-isopentenyladenosine

17

Indole-3-lactic acid

60

Gibberellin A7

103

Auxin

18

3-Indoleacrylic acid

61

Gibberellin A9

104

Brassinosteroid

19

N-(3-Indolylacetyl)-L-alanine

62

N-[(-)-Jasmonoyl]-(l)-phenalanine

105

Cytokinin

20

L-tryptophan

63

N-[(-)-Jasmonoyl]-(L)-valine

106

Ethylene

21

N-(3-Indolylacetyl)-L-phenylalanine

64

3-oxo-2-(2-(Z)-Pentenyl) cyclopentane-1-butyric acid

107

Gibberellins

22

Indole-3-carboxylic acid

65

3-oxo-2-(2-(Z)-Pentenyl)cyclopentane-1-hexanoic acid

108

Jasmonates

23

1-O-indol-3-ylacetylglucose

66

Dihydrojasmonic acid

109

Strigolactone

24

Indole-3-acetic acid

67

Jasmonic acid

110

Phytosulfokine

25

Indole-3-butyric acid

68

Jasmonoyl-L-isoleucine

111

Melatonin

26

Indole-3-carboxaldehyde

69

Methyl jasmonate

112

Kinetin

27

Methyl indole-3-acetate

70

cis(+)-12-Oxophytodienoic acid

113

Kinetin riboside

28

3-Indolepropionic acid

71

Salicylic acid

114

para-Topolin riboside

29

Dihydrozeatin ribonucleoside

72

Salicylic acid 2-O-β-glucoside

115

ortho-Topolin riboside

30

cis-Zeatin riboside

73

5-Deoxystrigol

116

cis-Zeatin-9-glucoside

31

4-[[(9-beta-D-Glucopyranosyl-9H-purin-6-yl)amino]methyl]phenol

74

(±)Strigol

117

N6-Isopentenyl-adenine-9-glucoside

32

2-Chloro-trans-zeatin

75

Abscisic aldehyde

118

N6-Isopentenyl-adenine-7-glucoside

33

para-Topolin

76

Indole

119

trans-Zeatin-O-glucoside

34

meta-Topolin

77

cis-Zeatin riboside monophosphate

120

Dihydrozeatin-O-glucoside riboside

35

meta-Topolin riboside

78

N-6-iso-pentenyladenosine-5'-monophosphate

121

cis-Zeatin-O-glucoside riboside

36

ortho-Topolin

79

trans-Zeatin-9-glucoside

122

meta-Topolin-9-glucoside

37

6-Benzyladenine

80

9-Ribosyl-trans-zeatin 5'-monophosphate

123

ortho-Topolin-9-glucoside

38

6-Benzyladenosine

81

Gibberellin A44

124

N6-Benzyladenine-9-glucoside

39

Gibberellin A51

82

Gibberellin A8

125

N6-Benzyladenine-7-glucoside

40

Gibberellin A12 aldehyde

83

Gibberellin A5

126

Jasmonate-1-aminocyclopropane-1-carboxylic acid

41

12-Hydroxyjasmonic acid

84

Gibberellin A6

127

2-Methoxycarbonylphenyl beta-D-glucopyranoside

42

L-Phenylalanine

85

Gibberellin A29

128

Melatonine

43

trans-Cinnamic acid

86

Gibberellin A34

129

2-Coumarate

*All analytes and Internal Standards are measured by NMR and ACQUITY UPLC/TripleQuad5500 (Waters/AB Sciex)

For other plant hormone analysis services, please feel free to contact us.

 

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Protein Analysis

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Protein Mass Measurement

PTMs Identification

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