LC-MS/MS-Based Exosome Proteomics Workflow: Isolation, Digestion, Acquisition, and Analysis
Exosome proteomics by LC-MS/MS links extracellular vesicle biology to biomarkers and disease pathways when isolation, digestion, acquisition, and annotation are aligned.
Key Takeaways
- Sample type sets sensitivity and contamination risk.
- Isolation trades purity, throughput, and yield.
- Controlled digestion matters for low-input vesicle protein.
- DDA, DIA, and PRM serve discovery, cohort quant, and validation.
- Use ExoCarta/Vesiclepedia to support vesicle specificity.
Related Services
Active Exosome Isolation Service
Animal Cell-derived Exosome Isolation and Development Service
Exosomal Peptidomics Detection Service
Protein Extraction through LC-MS/MS
Lyse, quantify, reduce/alkylate, trypsin digest, desalt, optional fractionation, nanoLC, then DDA, DIA, or PRM as study design requires.
Data Analysis
Search at <1% FDR; differential screening; GO/KEGG/PPI; ExoCarta and Vesiclepedia cross-checks.
FAQ
Can exosome proteomics use DIA?
Yes, for reproducible multi-sample quantification with suitable libraries.
Conclusion
Document each workflow step to move from vesicle profiles to translational insight.
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