Exosome Proteomics: Overview and Workflow

Exosome proteomics profiles proteins in 30-150 nm extracellular vesicles from biofluids and cell culture, supporting liquid biopsy, signaling studies, and biomarker discovery.

Key Takeaways

EV cargo reflects parent-cell state and disease biology.
Isolation (UC, SEC, immunocapture) sets MS specificity.
Quantification options include DIA, TMT, and label-free LC-MS/MS.
Bioinformatics links proteins to pathways and EV databases.

Exosome isolation — Figure 1. Match isolation stringency to downstream MS.

Exosome MS quant — Figure 2. Choose quant strategy by cohort design.

Related Services

Exosome Proteomics Service

Exosomal Proteomic Quantitative Service

Exosome Quantitative Proteomics Service

Applications — Figure 3. Oncology, neuro, and immune applications.

FAQ

1. How pure should EV prep be?

Increase stringency (SEC, beads) when serum protein carryover limits IDs.

2. DIA or label-free?

DIA for reproducible cohorts; label-free for flexible designs.

Conclusion

Exosome proteomics connects vesicle biology to measurable protein signatures in accessible fluids.

Submit Inquiry

How to order?

How to order