Chemoproteomics: Principles, Workflow, and Applications

Workflow: design selective probes → label live lysates → enrich tagged proteins → LC-MS/MS → map targets and networks.

Key Takeaways

• ABPP captures active enzyme pockets.

• Target ID maps drug binders.

• Controls reduce non-specific labeling.

• MS delivers sensitivity at proteome scale.

Figure 1. Probe labeling workflow.

Related Services

Chemical Proteomics Analysis Service

Activity-Based Protein Profiling (ABPP) Service

Target Identification Service

Protein Identification by LC-MS/MS Service

FAQ

1. How is chemoproteomics different from standard proteomics?

It prioritizes chemical engagement and activity, not abundance alone.

2. What controls are needed?

Competition with free inhibitor and minus-probe controls.

Conclusion

Chemoproteomics reveals functional protein states liganded by small molecules.