Chemoproteomics: Principles, Workflow, and Applications
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ABPP captures active enzyme pockets.
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Target ID maps drug binders.
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Controls reduce non-specific labeling.
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MS delivers sensitivity at proteome scale.
Workflow: design selective probes → label live lysates → enrich tagged proteins → LC-MS/MS → map targets and networks.
Key Takeaways
Figure 1. Probe labeling workflow.
Related Services
Chemical Proteomics Analysis Service
Activity-Based Protein Profiling (ABPP) Service
Protein Identification by LC-MS/MS Service
FAQ
1. How is chemoproteomics different from standard proteomics?
It prioritizes chemical engagement and activity, not abundance alone.
2. What controls are needed?
Competition with free inhibitor and minus-probe controls.
Conclusion
Chemoproteomics reveals functional protein states liganded by small molecules.
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