Antibody Characterization Guide: Sample Preparation, Analytical Methods, and Data Interpretation

Systematic antibody characterization documents sequence, purity, modifications, binding, and stability before research or therapeutic use.

Key Takeaways

• Confirm sequences by MS or cDNA when needed.
• SEC-HPLC and CE-SDS quantify aggregates and fragments.
• Glycan and PTM mapping affect Fc function.
• SPR/BLI measures binding kinetics.
• DSC and DLS assess stability.

Related Services

Antibody Characterization Service

Antibody Sequencing Service | Mass spectrometry

De Novo Antibody Sequencing Service

Antibody CDR Sequencing Service

Core Workflow

Sequence confirmation, purity analysis, glycosylation/PTM mapping, binding assays, and stability testing form the standard path.

Challenges

Combine SEC-HPLC with DLS for aggregation; use LC-MS/MS for PTMs; de novo sequencing for unknown commercial antibodies.

Conclusion

Structured characterization reduces failed experiments and supports confident antibody programs.