Amino Acid Analysis Service

Published: April 15, 2024

Amino acids are fundamental organic compounds with amino and carboxyl groups, serving as the building blocks of proteins and peptides. While approximately 20 amino acids are commonly known for protein synthesis, many more exist in nature. These compounds are essential for various biological processes, including protein synthesis, nitrogen balance, and metabolic regulation. Recent studies highlight their role in regulating glucose and lipid metabolism, maintaining homeostasis, and influencing gene expression, immune responses, and disease development.

MtoZ Biolabs offers amino acids analysis service using GC/MS (Agilent 7890A/5975C) and LC/MS/MS (AB Sciex 5500), which has characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of amino acid metabolites even in low abundance. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be greatly reduced, thus further increasing detecting sensitivity.

Table 1. List of Amino Acids Detecteble by MtoZ Biolabs

NO.	Metabolite	NO.	Metabolite	NO.	Metabolite
1	L-Histidine	43	2-Aminoethanesulfonic Acid	85	Glycyl-L-Proline
2	L-Arginine	44	L-Sarcosine	86	Trimethylamine N-Oxide
3	L-Serine	45	1,3-Dimethyluric Acid	87	1,3,7-Trimethyluric Acid
4	Glycine	46	N-Propionylglycine	88	3-Hydroxyhippuric Acid
5	L-Asparagine	47	N-Isovaleroylglycine	89	N8-Acetylspermidine
6	L-Glutamic Acid	48	Succinic Acid	90	(S)-β-Aminoisobutyric Acid
7	L-Threonine	49	5-Hydroxy-Tryptophan	91	S-Sulfo-L-Cysteine
8	L-Alanine	50	3,7-Dimethyluric Acid	92	Methionine Sulfoxide
9	L-Proline	51	L-Aspartate	93	Nα-Acetyl-L-Arginine
10	L-Lysine	52	L-Asparagine Anhydrous	94	1-Methylhistidine
11	L-Cystine	53	5-Hydroxy-Tryptamine	95	γ-Glutamate-Cysteine
12	L-Tyrosine	54	L-Homocitrulline	96	Nα-Acetyl-L-Glutamine
13	L-Methionine	55	Beta-Alanine	97	N-Acetyl-L-Tyrosine
14	L-Valine	56	Sarcosine	98	γ-Aminobutyric Acid
15	L-Isoleucine	57	L-Pipecolic Acid	99	D-Alanyl-D-Alanine
16	L-Leucine	58	L-Theanine	100	Guanidinoethyl Sulfonate
17	L-Phenylalanine	59	Cystathionine	101	Homo-L-Arginine
18	L-Tryptophan	60	3-N-Methyl-L-Histidine	102	L-Tryptophyl-L-glutamic Acid
19	L-Cysteine	61	Homoserine	103	Nicotinuric Acid
20	L-Glutamine	62	3-Iodo-L-Tyrosine	104	N-Acetylneuraminic Acid
21	1-Methyl-L-Histidine	63	P-Aminohippuric Acid	105	N,N-Dimethylglycine
22	3-Methyl-L-Histidine	64	4-Acetamidobutyric Acid	106	N-Acetylaspartate
23	L-Hydroxyproline	65	α-Aminoadipic Acid	107	L-Homocystine
24	O-Phosphorylethanolamine	66	D-Homocysteine	108	3-Aminoisobutanoic Acid
25	L-Carnosine	67	Trans-4-Hydroxy-L-Proline	109	Glutathione Oxidized
26	L-Anserine	68	Creatine	110	L-α-Aspartyl-L-Phenylalanine
27	Taurine	69	Kynurenine	111	N-Glycyl-L-Leucine
28	Ethanolamine	70	L-Cystathionine	112	Creatine Phosphate
29	L-Citrulline	71	5-Aminovaleric Acid	113	3-Chloro-L-Tyrosine
30	β-Alanine	72	N6-Acetyl-L-Lysine	114	S-(5-Adenosy)-L-Homocysteine
31	L-α-Aminobutyric Acid	73	Phosphorylethanolamine	115	Kynurenic Acid
32	D,L-β-Aminoisobutyric Acid	74	L-tyrosine methyl Ester	116	N'-Formylkynurenine
33	γ -Aminobutyric Acid	75	2-Aminobutyric Acid	117	Glycylphenylalanine
34	δ-D,L-Hydroxylysine	76	(5-L-Glutamyl)-L-Amino Acid	118	Argininosuccinic Acid
35	L-α-Aminoadipic Acid	77	Anserine	119	5-Hydroxylysine
36	L-Ornithine	78	6-Aminocaproic Acid	120	O-Phospho-L-Serine
37	Urea	79	Threonine	121	L-4-Hydroxyproline
38	Glutamine	80	Lysine	122	Tyrosine
39	Proline	81	Phenylalanine	123	Serine
40	Histidine	82	Ornithine	124	Valine
41	Arginine	83	Tryptophan	125	Citrulline
42	Catecholamine	84	Methionine

Note: The table above shows only a selection of the amino acids we can analyze. For other amino acid analysis services, please feel free to contact us.

Analysis Workflow

Sample Submission Requirements

1. Sample Type and Volume

For other sample types and amounts, please contact MtoZ Biolabs.

2. Sample Transportation

Calculate with 3-4 kg of dry ice evaporating per day, please use a sufficient amount of dry ice for transportation (it is recommended to use larger pieces of dry ice, as larger pieces evaporate more slowly), and seal with a foam box. Also, try to choose a faster mailing method to reduce the possibility of sample degradation during transportation.