How to Use GFP Tags for Co-Immunoprecipitation?
- Tag position affects function, test N- or C-terminal fusions.
- GFP-Trap gives high-affinity capture.
- Reduce background with extra washes and mild detergent.
- Co-IP/MS scales to hundreds of interactors.
Express GFP fusion, lyse gently at 4°C with inhibitors, incubate GFP-Trap beads, wash stringently, elute for Western blot or LC-MS/MS interaction mapping.
Key Takeaways
Related Services
Co-Immunoprecipitation Protein Interaction Analysis Service
Protein Interaction Analysis Service
Protein-Protein Interaction Analysis Services
FAQ
1. GFP vs epitope tags?
GFP allows fluorescence tracking plus universal capture reagents.
2. Low capture efficiency?
Check fusion integrity and tag placement.
Conclusion
GFP Co-IP is a versatile entry point to validate and discover protein interaction partners.
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