How to Use GFP Tags for Co-Immunoprecipitation?

Express GFP fusion, lyse gently at 4°C with inhibitors, incubate GFP-Trap beads, wash stringently, elute for Western blot or LC-MS/MS interaction mapping.

Key Takeaways

• Tag position affects function, test N- or C-terminal fusions.
• GFP-Trap gives high-affinity capture.
• Reduce background with extra washes and mild detergent.
• Co-IP/MS scales to hundreds of interactors.

Related Services

Co-Immunoprecipitation Protein Interaction Analysis Service

Protein Interaction Analysis Service

Protein-Protein Interaction Analysis Services

FAQ

1. GFP vs epitope tags?

GFP allows fluorescence tracking plus universal capture reagents.

2. Low capture efficiency?

Check fusion integrity and tag placement.

Conclusion

GFP Co-IP is a versatile entry point to validate and discover protein interaction partners.